Which infectious agent is the most resistant to sterilization?

bacteria
virus
fungi
prion
The smaller the agent, the more difficult it is to destroy it. Prions are quite resistant to proteases, heat, radiation, and formalin treatments. 134°C (274°F) for 18 minutes in a pressurized steam autoclave may not be enough to deactivate the agent of disease. Prions may last for decades in the environment.
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Answers by country

bacteria
virus
fungi
prion
answers
flagGermany
5%
11%
6%
76%
138
flagRepublic of Ireland
4%
23%
1%
68%
63
flagPakistan
13%
13%
7%
65%
134
flagItaly
18%
14%
3%
62%
27
flagthe Netherlands
10%
18%
10%
60%
38
flagPoland
9%
20%
10%
59%
20476
flagGreece
10%
17%
14%
57%
28
flagAustralia
7%
18%
14%
53%
76
flagUnknown
14%
20%
16%
50%
50
flagMalaysia
15%
13%
20%
50%
44
flagthe United Kingdom
15%
25%
10%
47%
367
flagCanada
14%
26%
10%
47%
95
flagthe United States
11%
27%
13%
44%
1017
flagthe United Arab Emirates
17%
23%
11%
44%
34
flagSingapore
24%
28%
4%
44%
25
flagSouth Africa
15%
27%
9%
42%
33
flagIndia
18%
27%
10%
40%
920
flagGhana
13%
47%
0%
34%
38
flagthe Philippines
26%
23%
18%
29%
117
flagNigeria
11%
41%
16%
28%
81