Which infectious agent is the most resistant to sterilization?

bacteria
virus
fungi
prion
The smaller the agent, the more difficult it is to destroy it. Prions are quite resistant to proteases, heat, radiation, and formalin treatments. 134°C (274°F) for 18 minutes in a pressurized steam autoclave may not be enough to deactivate the agent of disease. Prions may last for decades in the environment.
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Answers by country

bacteria
virus
fungi
prion
answers
flagGermany
2%
14%
2%
80%
70
flagPakistan
11%
13%
7%
66%
118
flagItaly
19%
9%
4%
66%
21
flagRepublic of Ireland
8%
25%
2%
60%
35
flagPoland
9%
20%
11%
57%
18365
flagGreece
8%
17%
17%
56%
23
flagAustralia
5%
19%
13%
54%
51
flagUnknown
14%
20%
16%
50%
50
flagMalaysia
9%
15%
25%
50%
32
flagthe United Arab Emirates
12%
25%
12%
50%
24
flagthe United Kingdom
14%
25%
10%
48%
252
flagthe United States
11%
27%
13%
44%
852
flagIndia
17%
27%
9%
43%
689
flagthe Netherlands
17%
26%
13%
43%
23
flagSouth Africa
15%
26%
5%
42%
19
flagSingapore
26%
26%
5%
42%
19
flagCanada
15%
31%
12%
40%
66
flagGhana
15%
42%
0%
38%
26
flagNigeria
0%
47%
13%
34%
23
flagthe Philippines
25%
22%
18%
32%
94