Which infectious agent is the most resistant to sterilization?

bacteria
virus
fungi
prion
The smaller the agent, the more difficult it is to destroy it. Prions are quite resistant to proteases, heat, radiation, and formalin treatments. 134°C (274°F) for 18 minutes in a pressurized steam autoclave may not be enough to deactivate the agent of disease. Prions may last for decades in the environment.
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Answers by country

bacteria
virus
fungi
prion
answers
flagGermany
3%
13%
3%
79%
58
flagItaly
16%
5%
0%
77%
18
flagPakistan
11%
11%
8%
68%
110
flagPoland
9%
21%
11%
57%
17672
flagGreece
10%
15%
20%
55%
20
flagRepublic of Ireland
8%
34%
0%
52%
23
flagUnknown
14%
20%
16%
50%
50
flagMalaysia
9%
15%
25%
50%
32
flagthe United Kingdom
14%
25%
10%
48%
225
flagAustralia
7%
24%
12%
48%
41
flagthe United Arab Emirates
13%
26%
13%
47%
23
flagthe United States
11%
27%
13%
44%
819
flagIndia
16%
27%
9%
44%
610
flagthe Netherlands
15%
26%
15%
42%
19
flagSingapore
26%
26%
5%
42%
19
flagCanada
16%
33%
11%
38%
62
flagSouth Africa
16%
27%
5%
38%
18
flagGhana
16%
41%
0%
37%
24
flagNigeria
0%
52%
11%
35%
17
flagthe Philippines
24%
22%
18%
34%
86